A human monoclonal antibody specific for tetanus toxoid

Successful hybridization has been obtained between PWM stimulated peripheral blood lymphocytes from tetanus toxoid immunized donors and the human B lymphoma line RH-L-4. Among the hybrids selected after cloning, the average in vitro activity against tetanus toxoid was 1-5 micrograms/10(6) cells/24 hours. The specific activity of one of them was further tested in vitro (RIA) and in vivo (mouse protection test) and compared favourably with a human polyclonal antitetanus globulin.