Preparation of MODS rabbit model induced by hemorrhage with endotoxin

Objective To establish a standard rabbit model that would imitate the clinical features of multiple organ dysfunction syndrome(MODS) induced by hemorrhagic shock combined with endotoxin.Methods A rabbit model of hemorrhagic shock was established by bleeding and maintaining blood pressure within 40mmHg for 1h,followed by rapid resuscitation with Ringer's lactate and shed blood.Then rabbits were divided into 4 groups in random and subjected to different dose of endotoxin(LPS) to determine the dose to cause MODS.The parameters of important organ functions were measured,changes of pathology were observed by microscopy,and TNF-α,IL-6 were studied by ELISA.Results The animals in 10μg/kg LPS injection group all presented the characteristic systemic imflammatory response syndrone (SIRS) and the mortality rate was parallel to the clinical situation.Occurrence of SIRS,significant increase of organ function parameters and changes of pathology further confirmed that MODS was induced through this method.Conclusion This model replicated well the clinical characteristics of MODS associated with hemorrhagic shock followed by endotoxin.