The role of miRNA-339-5p in the function of vascular endothelial progenitor cell in patients with PCOS

Abstract Research Question miRNA-339 participates in diseases with endothelial progenitor cells (EPCs) dysfunction. The aim of this study was to explore the role of miRNA-339-5p in EPCs of polycystic ovary syndrome (PCOS). Design Clinical data of 76 controls and 84 PCOS patients were collected. Noradrenaline, asymmetric dimethylarginine (ADMA), advanced glycation end products (AGEs), silent information regulator 1 (SIRT1) in the serum were measured. The functions of EPCs and the expressions of PI3K, AKT, SIRT1, PGC-1α in EPCs before and after transfected with miRNA-339-5p analogue or miRNA-339-5p inhibitor were compared. Results The serum levels of noradrenaline, ADMA and AGEs were significantly higher and the SIRT1 level was significantly lower in PCOS patients especially obese ones than that in control group (p 0.05), the migration and tubular formation were significantly decreased (p Conclusion Increased sympathetic excitation and damage of EPCs were observed in PCOS patients, especially those obese ones. Up-regulated miRNA-339-5p could inhibit the function of EPCs through inhibiting PI3K/AKT and SIRT1/PGC-1α signaling pathways.