Évaluation du CDx90® (Microgenics Thermo Fisher Scientific) pour le dosage urinaire des drogues

Summary Objective The aim of the study is to assess the CDx90 ® , the new automated biochemistry analyzer by Microgenics Thermo Fisher Scientific for the measurement of urinary illicit drugs: EDDP (2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine), cocaine, opiates, amphetamines and benzodiazepines. Material and methods Hundred and fifteen urine samples from toxicomans treated by methadone were analysed with the CDx90 ® and CEDIA reagents. Results were compared with those obtained with a Cobas Mira ® (Roche) and a DxC600i ® (Beckman-Coulter) for benzodiazepines. Analytical performances of the CDx90 ® were evaluated according to the French Society of Analytical Toxicology (SFTA) with a two-point calibration: intra-assay ( n  = 21 repeats) and inter-assay precision ( n  = 12 repeats) with two control levels (hight and low), limits of detection and of quantification (negative urine sample; n  = 21 repeats). Results Coefficients of variation are very acceptable (intra-assay less than 5% and inter-assay precision less than 10%, for the cocaine less than 12%). Limits of detection and quantification were comprised between 3,7 and 32,8% of positive cut-offs. The concordance between CDx90 ® , Cobas Mira ® and DxC600i ® is excellent, particularly 100% for opiates, cocaine and EDDP. The CDx90 ® appears as a suitable analyzer to replace the Cobas Mira ® for measurement of urinary illicit drugs.