Effect of NDRG2 siRNA sensitivity of cervical cancer Hela cells to cisplatin

OBJECTIVE:To explore the effects of small interfering RNA(siRNA)specific to NDRG2 on chemosensitivity to cisplatin of cervical cancer Hela cells.METHODS:The siRNA specific for NDRG2 was synthesized and transfected into cervical cancer Hela cells by lipofectamine.NDRG2 expression in Hela cells was detected by RT-PCR and Western blot.MTT assay was used to determine the inhibitory effects of cisplatin combined with NDRG2 specific siRNA in vitro.RESULTS:RT-PCR and Western blot analysis demonstrated that Hela cells transfected with the NDRG2 siRNA oligomer significantly reduced NDRG2 transcript and protein levels.It increased the inhibitory effects of cisplatin to Hela cells viability in vitro.The IC50 for cisplatin in Hela-negative control cells was(6.69±0.33)μg·L-1 but was decreased almost 4-fold to(1.69±0.25)μg·L-1 in Hela-NDRG2-HSS126269 siRNA cells.Statistical analysis showed the difference of the IC50values between the two groups to be highly significant(t=17.311,P=0.003).CONCLUSION:Downregulation of NDRG2 results in increased sensitivity to cisplatin in cervical cancer Hela cells and can be used as a powerful adjunct to conventional chemotherapy.