Construction of a semisynthetic phage antibody library

Objective To construct a semisynthetic phage antibody library for by passing immunization. Methods The genomic V H segment genes were amplified from fetus liver cell DNA and spliced to synthetic random CDR3 of different lengths, forming an Fd library. This Fd library was then combined with a human κ gene library to form a semisynthetic phage antibody library. Results An antibody library containing 4×10 8 different clones was obtained, with more than 90% containing Fd and κ chain genes. 50% of the clones expressed Fab fragment. Specific Fab could be selected after panning against 3 different antigens. Conclusion This semisynthetic antibody library is capable of cloning antibodies by passing immunization.