Solid-phase synthesis and immunoreactivity of penta-O-(N-acetyl-α-D-galactosaminyl)-MUC1 eicosapeptide, a glycosylated counter part of the highly immunogenic tandem repeat sequence of carcinoma-associated mucin

Abstract Penta- O -glycosylated MUC1 peptide HVal-Thr * -Ser * -Ala-Pro-Asp-Thr * -Arg-Pro-Ala-Pro-Gly-Ser * -Thr * -Ala-Pro-Pro-Ala-His-Gly-OH ( * α- D -GalNA cp ) ( 2 ) has been synthesized by a solid-phase method on Sasrin resin using Fmoc-glycoaminoacids 3 and 4 as building blocks. The monoclonal antibodies SM3 and HMPV, specific to the MUC1 peptide 1 , also react strongly with 2 .