Fluorescence and multilayer structure of the scorpion cuticle

We collect the scorpions, Isometrus maculates, in different instars to analyze the photoluminescence (PL), micro‐structure of cuticles and their correlation. The photoluminescence is excited by 405 nm solid laser in room temperature and detected by BWtek BRC 112E spectrometer. The result shows that the intensity of photoluminescence positively correlate to instars of scorpion. The images of micro‐structures of cuticles captured by scanning electron microscope (SEM) present the multilayer structure in detail. The samples are prepared in small piece to ensure that the PL and SEM data are caught from the same area. The correlation between instars and intensity of photoluminescence is explained according to micro‐structures via the thin‐film optics theory.