Impedimetric aptasensor for determination of the antibiotic neomycin B

Abstract Electrochemical impedance spectroscopy is a powerful electrochemical technique for studying electrode–solution interfaces with applications in different fields such as energy (batteries), corrosion, and bioanalysis. When applied to report biomolecular recognition events (antigen–antibody, DNA hybridization, aptamer–ligand), it simplifies the operational procedure, although parallel tests for ensuring the specificity of the detection are compulsory. This experiment proposes the development of a label-free sensor for the detection of neomycin B based on a displacement bioassay using faradaic impedance spectroscopy, showing the usefulness of this technique in bioanalysis.