Detection of anti-non structural protein antibodies against foot-and-mouth disease virus in the bovine population of Haryana during FMD control programme in the year 2012.

The non structural protein ELISA (NSP-ELISA) was used to detect the antibodies against 3AB3 NSP of foot-and-mouth disease virus (FMDV) in the bovine serum samples collected from different districts of Haryana. The NSP-ELISA as developed and standardized using E. coli expressed recombinant r3AB3 by the Project Directorate on FMD, Mukteswar, Uttarakhand was used in the present study. Serum samples from a total of 4200 randomly selected animals from all the 21 districts (200 from each district, 100 each from cattle and buffalo) of Haryana [eight under FMD-Control Programme (FMD-CP, launched in 2003-04) Pre-Phase XV and 13 under extended FMD-CP, launched in 2010-11, Pre-Phase V] were analyzed. Of the 4200 serum samples, 76 (1.809%) serum samples were found positive for anti-3AB3 NSP antibodies. The serum samples from eight FMD-CP districts of Haryana demonstrated anti-3AB3 NSP FMDV antibodies in 3% (48/1600) animals. On the other hand, the serum samples from extended FMD-CP districts demonstrated anti-3AB3 NSP antibodies in 1.077% (28/2600) animals. Higher virus activity was recorded in Bhiwani, Jhajjar and Sirsa (all FMD-CP districts). Significantly higher proportion of cattle (2.85%; 60/2100) had r3AB3 specific antibodies compared to buffaloes (0.76%; 16/2100).