Vomitoxin hybridoma, monoclonal antibody, and preparation method and application of monoclonal antibody

The invention discloses vomitoxin hybridoma, a monoclonal antibody, and a preparation method and application of the monoclonal antibody. The preparation method comprises the steps of: immunizing a Balb/c mouse with a vomitoxin and bovine serum albumin conjugate as an immunizing antigen; preparing a hybridoma by the spleen cells and the myeloma cell SP2/0 of the mouse; obtaining a monoclonal antibody hybridoma strain D-2-1CGMCC No. 5161 through an indirect ELISA (Enzyme-Linked Immunosorbent Assay) screening and a limiting dilution method; and injecting the hybridoma strain into the abdominal cavity of the mouse to produce a monoclonal antibody, wherein the monoclonal antibody is used for indirect competitive ELISA testing of the vomitoxin. The monoclonal antibody is capable of specifically detecting the vomitoxin, and high in sensitivity; and when an ELISA kit prepared by the monoclonal antibody is used for detecting the vomitoxin, the cost is low, large equipment is not needed, the operation is simple and convenient, and the preparation method is convenient for popularization and utilization.