Combination of several bioinformatics approaches for the identification of new putative glycosyltransferases in Arabidopsis.
2009
Approximately 450 glycosyltransferase (GT) sequences have been
already identified in the Arabidopsis genome that organize into
40 sequence-based families, but a vast majority of these gene
products remain biochemically uncharacterized open reading
frames. Given the complexity of the cell wall carbohydrate
network, it can be inferred that some of the biosynthetic genes
have not yet been identified by classical bioinformatics
approaches. With the objective to identify new plant GT genes,
we designed a bioinformatic strategy that is based on the use
of several remote homology detection methods that act at the
1D, 2D, and 3D level. Together, these methods led to the
identification of more than 150 candidate protein sequences.
Among them, 20 are considered as putative glycosyltransferases
that should further be investigated since known GT signatures
were clearly identified.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
58
References
26
Citations
NaN
KQI