ACTIVITY OF DEHYDROGENASES AND ENZYMES OF NITROGEN METABOLISM IN RUMEN MUCOSA AND LIVER OF STEERS FED . MONENSIN

Kalal!njuk G. I., M. Marounek, M. G. Gerasymiv, O. G. Savka, L. G. Kalal!njuk: Activity of Dehydrogenases and Enzymes of Nitrogen Metabolism in Rumen Mucosa and Liver of Steers Fed Monensin. Acta vet. Bmo, 62, 1993: 127-131. The effect of monensin on activity of intracellular enzymes of tissues was de­ termined in ten steers fed hay, silage and concentrate. Five steers received monensin in amount of 0.5 mg per 1 kg of live weight per day. Mter 10 months steers were slaughtered and activity of dehydrogenases and enzymes of nitrogen metabolism assayed in mitochondrial and cytoplasmic fraction of cells of rumen mucosa and liver. Monensin had no consistent effect on activity of nine enzymes examined (lactate de­ hydrogenase, malate dehydrogenase, ex-ketoglutarate dehydrogenase, glutamate dehydrogenase, glutamin synthetase, arginase, ornithine carbamoyltransferase, aspartate aminotransferase and alanine aminotransferase). The results indicate that long-term inclusion of monensin into diet of steers has no damaging effect on cells of rumen mucosa and liver.