Establishment and application of rapid detection method for influenza virus and subtypes based on flow fluorescence technology

Objective To develop a method for rapid detection of influenza virus and its subtypes based on flow fluorescence technology, and then detect the clinical specimens by our established method . Methods We designed degenerate primers and specific probes, synthesized plasmids, used Luminex platform to establish detection method and later detected 430 clinical specimens, and compared the result with those of Fujian Center for Disease Control and prevention. Results A method for the simultaneous determination of influenza viruses A, B, C and its subtypes (H116, N19) was established. The time consumption was 3.5 hours, with good specificity, high sensitivity and feasible stability. The detection result of 430 clinical specimens showed high consistency with the result of Fujian Center for Disease Control and Prevention. Conclusions We established a method for simultaneous determination of influenza viruses and its subtypes, high sensitivity, specificity and stability. Key words: Influenza virus; Subtypes; Genotyping; Flow fluorescence technology