Serum Katalaz, Miyeloperoksidaz ve Paraoksonaz 1 Üzerine Bazı Bitki Ekstraktlarının In Vitro Etkisi

Background: It is emphasized that natural plants rich in phenolic content can be used in traditional and complementary medicine for therapeutic purposes. For this purpose, effects of the water extracts of Artemisia absinthium, Ferula communis, Achillea millefolium, Equisetum arvense and Hypericum perforatum on the serum total anti-oxidant (TAS) level and total oxidant level (TAS) and paraoxanase 1 (PON1), myeloperoxidase (MPO) and catalase (CAT) enzyme activities has been investigated. Material and method: The water extract of each plant was prepared, and these extracts were added to the serum samples in a certain amount and the final concentration was applied as 60 µg/mL. TAS and TOS parameters were measured by Erel, MPO activity Bradley, PON1 activity Eckerson, CAT activity Goth method to investigate the effects of these prepared extracts on serum parameters. The reagents were prepared in Harran University Medical Faculty Medical Biochemistry Department Laboratory and analyzes were performed spectrophotometrically in the routine Biochemistry Laboratory autoanalyst (Architect C16000, Abbott). Results: Control values CAT (23,233 ± 1,665 kU/L), MPO (103,483 ± 4,048 U/L), PON1 (24,833 ± 1,404 U/L), TAS (1,223 ± 0.080 mmol Trolox Eqv/L) and TOS (6.350 ± 0,481 µmol H2O2 Eqv/L) was detected quantitatively. Artemisia absinthium, Ferula communis, Achillea millefolium and Hypericum perforatum inhibited CAT, MPO and PON1 enzymes (p <0.05, p <0.01). Equisetum arvense only showed an inhibition effect on MPO (p <0.01). Achillea millefolium and Hypericum perforatum decreased the TOS level, while increasing the TAS level (p <0.05, p <0.01). Conclusion: Ferula communis and Equisetum arvense plant extracts showed an inhibition effect on MPO activity in vitro, while a weak inhibition effect on PON1 activity. If the in vivo efficacy of these plants can be demonstrated, it can be said that in diseases such as atherosclerosis, it can inhibit plaque formation caused by intravascular lipid peroxidation and improve vascular functions. Key words: Medicinal Plant, Lipid peroxidation, Paraoxonase 1, Myeloperoxidase