Ischemic preconditioning protects against hepatic ischemia-reperfusion injury by attenuating leukotriene C4 production

AIM:To investigate the effect of ischemic preconditioning(IP) on leukotriene C4(LTC4) generation during hepatic ischemia-reperfusion(I/R) injury.METHODS:Adult male Sprague-Dawley rats were randomly divided into sham(control) group,I/R group and IP+I/R group.The rat liver was subject to 60 min of partial hepatic ischemia followed by 5 h of reperfusion.LTC4 content was measured by reversed-phase high-performance liquid chromatography(RP-HPLC).The activity of serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST) was determined,and the changes of histological structures were observed to assess the tissue injury.The reduced glutathione(GSH) level in the liver tissues was also examined by biochemical methods.RESULTS:IP markedly decreased LTC4 content in rat livers compared with I/R group.The activity of serum ALT and AST in I/R group and IP+I/R group was higher than that in control group.Compared with I/R group,the levels of serum ALT and AST were markedly decreased,and the level of GSH in hepatic tissues was elevated in IP+I/R group.In addition,the structural damage of the rat liver tissues in IP+I/R group displayed slighter than that in I/R group.CONCLUSION:IP treatment reduces LTC4 production accompanied with improving the liver function and histological structure during I/R injury,suggesting that the beneficial effects of IP may be involved in its repressing LTC4 generation during hepatic I/R injury.