Preparation, quality control and biological characterization of 99mTc-vincristine

In present study it is aimed to radiolabel vincristine with 99mTc and to evaluate bioaffinity of 99mTc labeled vinc. The optimum conditions required to obtain 99.6 ± 0.4 %, (n = 5) radiolabeling yield of 99mTc-vincristine (99mTc-vinc) were as follows: pH 4, 5 µg of vincristine sulphate, 6 µg SnCl2·2H2O as a reducing agent and 10 min incubation time at room temperature. Quality control of 99mTc-vinc was done by using paper electrophoresis and thin layer chromatography. The radiolabeling yield was confirmed by High performance liquid chromatography using radioactive and UV detector operating at 230 nm. 99mTc-vinc was stable in vitro for 5 h. Biodistribution and scintigraphy of 99mTc-vinc was performed in mice and rabbits respectively and that 99mTc-vinc showed high uptake of it in liver and spleen. Finally 99mTc-vinc may be the potential imaging agent for liver and spleen.