Study on the expression and relationship of p14~(ARF) protein and p53 protein in human glioma

Objective:To detect expression of p14ARF protein,p53 protein and explore three proteins′ relationship in the genesis and development of human glioma in different grade gliomas.Methods:Expression of p14ARF protein,p53 protein were detected by using S-P immunohistochemical technique in 31 cases of brain glioma with different pathological types and 5 cases of normal brain tissue.Results:The expression rate of p14ARF protein,p53 protein in low and high pathological grades was 75.00% and 30.43%(P0.05),12.50% and 69.57%(P0.05).A significant negative corelation(r=-0.571,P0.01) was observed between the p14ARF protein and pathological grades.On the contrary,mutant p53 protein showed a positive corelation with pathological grades(r=0.472,P≤0.01).The expression levels of p14ARF protein was higher with lower grade of malignancy.But the expression lever of p53 protein increased parallel with the elevation of glioma malignant degree.And there was a significant corelationship between the two kinds of proteins (P0.05).Conclusion:This study suggests that the expression of p14ARF protein and p53 protein play very important roles in the genesis and development of human brain glioma and they may coexist in it.The abnormal expression of the two proteins cause disorder of cell cycle modulation and increase of cellular proliferation activity which is an important factor to influence the biological character of brain glioma.