Design of Cyclic Peptides Based Glucose Receptors and Their Application in Glucose Sensing

Glucose assay is of great scientific significance in clinical diagnostics and bioprocess monitoring and to design new glucose receptor is necessary for development of more sensitive, selective and robust glucose detection techniques. Herein, a series of cyclic peptides (CPs) glucose receptor were designed to mimic the binding sites of glucose binding protein (GBP) and CPs’ sequence contained amino acid sites Asp, Asn, His, Asp, Arg, which constituted the first layer interactions of GBP. The properties of these CPs used as a glucose receptor or substitute for the GBP were studied by using quartz crystal microbalance (QCM) technique. It was found that CPs can be formed a self-assembled monolayer at Au quartz electrode surface and the monolayer’s properties was characterized by using cyclic voltammetry, electrochemical impedance spectroscopy and atomic force microscopy. The CPs’ binding affinity to saccharide (i.e., galactose, fructose, lactose, sucrose, and maltose) was investigated and the CPs’ sensitivity...