Effect of hepatocyte growth factor on rat submandibular gland cell proliferation

Objective To investgate the effect of hepatocyte growth factor on rat submandibular gland cell proliferation.Methods Submandibular gland cells were obtained and purified by amylase digestion from the gland of the rat. The epithelial cells were cultured and subcultured in 10% fetal bovine serum and various concentrations of hepatocyte growth factor on Dulbecco minimum essentia medium. MTT colorimetric method was used to assay numeric value and to estimate vital force of the cultured cells. The relations of dose response and time course were studied by MTT colorimetric method.Results Dose response:In Dulbecco minimum essentia medium containing 10% fetal bovine serum,hepatocyte growth factor (1.0 1?000?μg/L) stimulated the proliferation moderately (P0.01). In a definite range of hepatocyte growth factor (1.0,5.0,10.0,50.0,100.0?μg/L),hepatocyte growth factor had mitogenic effect dose dependently. Time course:The growth curve of experiment group moved to left and flat stage appeared in advance. Cell proliferation study indicated that hepatocyte growth factor had obvious promotion effect on cell proliferation.Conclusion Hepatocyte growth factor can promote the proliferation and differentiation of submandibular gland cells significantly in 10% fetal bovine serum conditions on Dulbecco minimum essentia medium.