Impedimetric quantification of the density of suspending Trichomonas vaginalis
2016
To investigate the chemosensitivity of T. vaginalis under tested drug, quantification of the density of T. vaginalis is necessary to study its viability. However, because T. vaginalis is suspended in the medium, microscopic quantification is not practical. In this work, impedimetric quantification of T. vaginalis was demonstrated using a biosensor. Investigations of impedance spectrum, sample loading volume, and correction between impedance magnitude and density of T. vaginalis were reported. Results showed the feasibility of the quantification of suspending cells based on impedance measurement technique. With the advantage of microfluidics technology, quantification of suspending cells can be completed in less sample volume (30 µl) and fast response (a few seconds). The present work has high potential to apply for the study of the development of therapeutic drug of T. vaginalis.
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