Metabolism of 3β,14α-dihydroxy-5β-(3α-3H)-cholest-7-en-6-one in Calliphora stygia

Abstract 3β,14α-Dihydroxy-5β-cholest-7-en-6-one (1) is metabolized in Calliphora stygia at the time of puparium formation via the ketotriol (10) and other hydroxylated derivatives to α-ecdysone (6) and β-ecdysone (7). The ketodiol (1) is thus a likely precursor of the ecdysones in Calliphora .