A study on detecting specific IgM in 426 cases of hemorrhagic fever with renal syndrome with colloidal golddot assay and treatment with traditional and western medicines

Objective To explore a simple，rapid specific and sensitive method to detect specific IgM（SIgM）and IgG（SIgG）antibody in hemorrhagic fever with renal syndrome（HFRS）．To study the therapeutic effects of traditional and western medicines on HFRS．Methods The serum of 426 patients with HFRS were tested with colloidal gold immuno-dot assay（CGIDA）for SIgM and SIgG antibody and compared with enzyme linked immunosorbent assay（ELISA）or indirect fluorescent antibody test （IFAT）and a control group（20 fever of unknown origin and 48 hepatic disorders ）．101patients with HFRS were randomized into a treatment group（n＝50，kuhuang and Shenmai injection with Huangqi liquid）and a control group（n＝51，Ribarvirin with Ganlixin injection）．Results With CGIDA，specificity for SIgM was 100% and sensitivity was 78.9%，specifiafy for SIgG was also 100% sensitivity was 90.8%．They were similar during the period of clinical improvement between treatment group and control group．Days for recovery from albuminuria and normalized kidney function，the treatment group did better than the control one（P＜0.05)．The rate of recovery from the shock state was higher the treatment group than that of the control one（P＜0.05）．Conclusion To detect the SIgM or SIgG antibody in serum of patients with HFRS，CGIDA was more simple，rapid，more specific and sensitive than ELISA or IFAT．Though the sensitivity of CGIDA was lower than that of ELISA，the CGIDA had no false positive reaction；the sensitivity of CGIDA was higher than of IFAT on detecting anti-HFRS IgG．Regarding treatment of HFRS，the effect of treatment group was similar to that of control group．But the rate of recovery from the shock state was higher in the treatment group than that of control，whereas normalization of kidney function in the control group was better than that in the treatment group．