Detection of DNA Repair Gene: ERCC2/XPD exon 6 Mutation from Human by Nested Polymerase Chain Reaction (NPCR)

BACKGROUND AIM: To study a method which can get ideal products of PCR in the sample of low yield DNA for mutation analysis. MATERIAL AND METHODS: The nested polymerase chain reaction(NPCR) -restriction fragment length polymorphisms (RFLP) technique was used for mutation test of single nucleotide polymorphism of DNA repair gene: ERCC2/XPD exon 6 in some samples of low yield DNA.RESULTS: Genotyping results of population were in agreement with the expectations of Hardy-Weinberg Equilibrium. CONCLUSION: The sophisticated new"nested PCR" systems are significantly more sensitive and specific than other currently availble PCR technologies.