In vitro fermentation of total mixed diets differing in concentrate proportion: relative effects of inocula and substrates
2016
BACKGROUND
In vitro techniques are used to predict ruminant feedstuff values or characterise rumen fermentation. As the results are influenced by several factors, such as the relative effects of inocula and substrates, this study aimed to examine in vitro incubation of two total mixed rations (substrates) differing in their proportion of concentrate [low (L): 350 g kg−1 vs. high (H): 700 g kg−1] incubated in inocula provided by goats fed either a L or a H diet. Gas production and composition in carbon dioxide (CO2), methane (CH4) and hydrogen (H2), volatile fatty acids (VFAs), soluble carbohydrates (SCs) and ammonia (NH3) concentrations, and pH of the fermentation fluid were measured.
RESULTS
In comparison with the L inoculum and L substrate, the H ones produced more CO2 and CH4 gas, which led to higher SCs and VFA concentrations, and lower acetate-to-propionate ratio and NH3 concentration, with a predominant effect of the inoculum.
CONCLUSION
The effects of the inocula and of the substrates were additive using donor animals adapted to the diets. © 2015 Society of Chemical Industry
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
27
References
7
Citations
NaN
KQI