Construction of recombinant expressing autoantigen SSA/Ro60

Objective:In view of constructing recombinant expressing plasmid of autoantigen SSA/Ro.Methods:The SSA/Ro60 cDNA was cloned by RT PCR and inserted into expression vector pGEX 2T.Results:The recombinant plasmid was identified by restriction enzyme digestion and DNA sequencing.Conclusion:The recombinant expressing plasmid of autoantigen SSA/Ro is useful for diagnosi.