Monochromatic He-Ne red light influence on callus tissue and buds explants

This paper presents some experimental results in order to give an interpretation of the action mechanism of laser radiation on biological tissue (especially vegetal tissue). Different types of woody plant tissue ( Sequoia sp. ) were irradiated with a low power 633 nm He-Ne laser beam. The laser power can be modified. The fresh weight of callus tissue increased about 70% as related to the untreated sample, when the laser's power was 10 mW. We observed that a 5 mW-laser power developed the bud differentiation. But, a significant promotion appeared for a higher laser powers (10 mW). The influence of laser radiation on the callus tissue growth process was detected also by the performing morphological and biochemical analysis.