Vector construction and high level expression of single chain immunotoxin against ovarian carcinoma

Objective: To construct an expression vector for co_expression of single chain Fv antibody (OC183B 2) and peudomonas exotoxin(PE38). Methods: Clone the genes of ScFv (OC183B 2) and PE38 into PET30a(+) and then express the immunotoxin after induced with IPTG. Results: Liable activity of antibody and toxic in were both observed in the fusion recombinant protein. Conclusion: The recombinant fusion protein 183B 2ScFvPE38 keeps the activity of both components and may be of great use in the future to deal with ovarian carcinoma.