Effect of Water Soluble Fraction of 0# Diesel Oil on Vitellogenin Level in Serum of Female Sebastiscus marmoratus

Female Sebastiscus marmoratus were exposed through the water to water soluble fraction of 0# diesel oil(DWSF) at 0.02,0.1,0.5 mg/kg,respectively.Serum testosterone levels decreased significantly,and serum 17β-estradiol and vitellogenin levels,which were analyzed by ELISA,decreased in the fish after exposure for 84 days compared with the corresponding controls.Estrogen receptor levels in the liver at the groups of DWSF exposure decreased compared with the corresponding controls analyzed by Western bolting.The decrease of 17β-estradiol and estrogen receptor levels would be principally causes of the decrease of vitellogenin,which would affect the development and quality of the ovum.