NEW POLYMERIC INHIBITOR OF GALACTOSYL TRANSFERASE

Abstract 2′,3′-Di-O-acetyluridine 5′-p-styrenesulfonate was synthesized by the reaction of 2′,3′-di-O-acetyluridine with p-styrenesulfonyl chloride and polymerized. After removal of acetyl groups, the polymeric product was shown by NMR spectroscopy and gel permeation chromatography to be poly(uridine 5′-p-styrenesulfonate). This uridine-containing polymer was tested against the galactosyl transferase that synthesizes lactose in the presence of α-lactalbumin. The polymeric compound did inhibit the enzyme with 75% inhibition requiring 120 μM which is only one percent of the concentration of glycosyl donor substrate (UDP-galactose, 12 mM). 1. Presented at the XVIIIth International Carbohydrate Symposium, Milan, Italy, July 21-26, 1996.