Analysis of genotoxicity of pymetrozine in human peripheral lymphocytes

Yildirim Z, Kayraldiz A, Donbak L, Kenger IH. Analysis of genotoxicity of pymetrozine in human peripheral lymphocytes. Adv Lab Med Int. 2017; 7: 30 - 42. ABSTRACT - Pymetrozine is an insecticide commonly used in aphid and whitefly struggle observed in citrus fruits and vegetables. In this study, possible genotoxic effects of pymetrozine were investigated in human peripheral blood lymphocytes by in vitro sister chromatid exchange (SCE), chromosome aberration (CA) and micronucleus (MN) tests and also cytotoxic effects of pymetrozine were determined with mitotic index (MI), proliferation index (PI), and nuclear division index (NDI) evaluations. The cells were treated with 5, 10, 20 and 40 μg/mL pymetrozine for 24 and 48 hours. SCE/Cell, CA/Cell, abnormal cell (AC), MN, and micronucleated binuclear cell (BNMN) frequencies were determined by microscopic analysis of the preparations. Obtained data were compared with the control group by using SPSS package programme (17.0). In the SCE frequency, there was a statistically significant increase only in the cells treated with the 20 µg/mL pymetrozine (p less than 0.05). Pymetrozine was markedly elevated the CA and AC incidences in the cells treated for 24 and 48 h as compared to control group (P less than 0.05). There was also a significant increase in both MN and BNMN frequencies of the cells treated with 20 μg/mL pymetrozine for 24 h and with tested doses for 48 h (except for 40 μg/mL) (p less than 0.05). Decrease in MI values was found to be significant at doses of 20 and 40 μg/mL for 24 h and 40 μg/mL for 48 h in comparison with the control (p less than 0.01). On the other hand, observed differences in PI and NBI values were found to be not significant (p more than 0.05). As a result; according to the data obtained from this study, pymetrozine had genotoxic effect and also weak cytotoxic effect in human peripheral lymphocytes. Full text available from Advance Laboratory Medicine Journal at https://drive.google.com/open?id=1cYKoRaRhZ3Iy0K7-S3Y7JygoecNEikRG