Immunomodulatory effect of characterized extract of Zataria multiflora on Th1, Th2 and Th17 in normal and Th2 polarization state

Abstract The effect of the extract of Zataria multiflora ( Z. multiflora ) on IFN-γ, FOXP3, IL-4, TGF-β, and IL-17 gene expression was evaluated in cultured splenocytes obtained from control, nontreated asthma or sensitized mice (group S), Sensetized animals treated with dexamethasone or three concentrations of Z. multiflora extract (200, 400 and 800 μg/ml) (n = 6, for each group). IFN-γ and FOXP3 gene expressions were significantly decreased ( P P P Z. Multiflora extract 800 μg/ml, significantly upregulated IFN-γ gene expression ( P P P Z. multiflora extract at all concentrations (200, 400 and 800 μg/ml) decreased TGF-β gene expression and its lowest concentration significantly reduced IL-17 gene expression compared to group S ( P P   0.001 for both cases). The results indicated an increase in IFN-γ and FOXP3 but decrease in TGF-β and IL-17 gene expression profile in sensitized splenocytes treated with the extract, which might be partially due to the presence of one of its constituent, carvacrol.