Effect of Insulin on Expression of Insulin Receptor in TC-3 Cell

Objective: To explore the effects of exogenous insulin on the expression of insulin receptors in mouse insulinoma TC-3 cells.Methods: The expression of insulin receptors in TC-3 cells in different incubation time(0 min,30mins,60mins,120mins,240mins) exposure to 100 IU/ml insulin was analyzed by immune fluorescence cytochemistry combined with laser scanning confocal microscopy.Semi-quantitative analysis of fluorescence intensity of insulin receptors was determined by Image Pro Plus 6.0 software.Results: The expression of insulin receptors at 30,60,120 and 240 min was decreased significantly compared with that of 0 min incubated with 100 IU/ml insulin(P0.05).Conclusion: The expression of insulin receptor in βTC3 cells was obviousiy decreased with high concen-tration insulin incubation,which may be one of the mechanism of insulin resistance caused by hyperinsulinemia.