Caracterização molecular e avaliação da formação e tolerância a biocida do biofilme de Pseudomonas aeruginosa isoladas de águas minerais naturais

Pseudomonas aeruginosa is an opportunistic pathogen associated with waterborne diseases and has the ability to form biofilms on surfaces. In this study, six samples of P. aeruginosa isolated from mineral waters in 20 L mineral water returnerd plastic bottled were subjected to molecular characterization by ERIC-PCR and evaluating biofilm formation. ERIC-PCR revealed that six strains detected distinct genetic genes. No strain shows biofilm formation in the stainless steel matrix. In the polystyrene matrix, biofilm was formed and exposure to sodium hypochlorite in the ranges of 1% and 2% eliminated the biofilm formed. However, at a concentration of 0.02% in the biofilm formed, it was not eliminated after 60 minutes of contact. The consumption of mineral waters contaminated by P. aeruginosa and the possibility of biofilm formation in plastic waterer can represent a risk for individuals belonging to the risk group, such as pregnant women, immunosuppressed and elderly people.