Biogenesis of 30-S subunits during recovery from inhibition of protein synthesis

Abstract The rate of synthesis of ribosomal proteins was determined early during recovery from (i) puromycin treatment, (ii) chloramphenicol treatment, and (iii) from methionine starvation. Patterns of ribosomal protein synthesis were similar, but not identical during recovery of the cells from the different treatments. Early during recovery production of several proteins is significantly increased while synthesis of another group is significantly reduced. Normal patterns of ribosomal protein synthesis were observed after 60–65 min of recovery from chloramphenicol treatment. At 20–25 min following recovery the proteins whose synthesis were elevated early were decreased; on the other hand, synthesis of those proteins which were depressed early during recovery were elevated. The results are interpreted to suggest that there is unbalanced accumulation of information for synthesis of 30-S ribosomal protein.