The optimization of technological processes, stability and microbiological evaluation of innovative natural ingredients-based multiple emulsion.

AbstractFor the last couple of decades, multiple emulsions were prepared either by the re-emulsification of primary emulsion or they were produced by an emulsion inversion and their technological peculiarities were widely investigated. The aim of our study was to investigate and determine the optimal technological parameters of innovative multiple emulsion, prepared directly—by addition of ethanolic rosemary extract in the presence of polymeric emulsifier—and evaluate its stability by experimental surface response design approach. The results revealed that simplified W/O/W emulsification process is stirring time and stirring speed sensitive: the change of stirring time from 5 to 15 min at 600 rpm resulted in increased viscosity (from 1705.6 ± 62.2 to 3364.1 ± 112.5 mPA/s) and smaller oil droplet size (from 33.09 ± 1.51 to 17.81 ± 0.78 μm), though the conductivity increased from 800 ± 2 to 882 ± 2 μS/cm (p < .05). The second mixing stage (1000 rpm) had a negative effect on the conductivity of W/O/W emulsio...