FARE AKCİGER GELİSİMİNDE GLUKOKORTİKOİDLERİN FİBRONEKTİN, KADERİN VE BETA-KATENİN MOLEKÜLLERİNİN DAGILISLARI ÜZERİNE ETKİSİ - THE EFFECTS OF GLUCOCORTICOIDS ON THE DISTRIBUTION OF FIBRONECTIN, CADHERIN AND BETA-CATENIN MOLECULES DURING MOUSE LUNG DEVELOPM

FARE AKCIGER GELISIMINDE GLUKOKORTIKOIDLERIN FIBRONEKTIN, KADERIN VE BETA-KATENIN MOLEKULLERININ DAGILISLARI UZERINE ETKISI Memeli akciger gelisimi hucreler ile ekstraselluler matriksler arasinda gerceklesen kompleks etkilesimleri icerir. Bu surec, fibronektin, laminin, kaderin, katenin ve kollajen gibi bir dizi matriks bileseni ve adezyon molekulu tarafindan duzenlenmektedir. Glukokortikoidler fotal akciger gelisiminde merkezi bir role sahiptir. Ornegin, gebelik sirasinda glukokortikoid uygulamasinin akciger gelisiminde degisiklige neden oldugu bilinmektedir. Bu calismada, eksojen glukokortikoidlerin fibronektin, kaderin ve beta-katenin molekullerinin gelisimsel profili uzerine etkilerini belirlemek icin immunohistokimyasal bir yontem uygulandi. Deksametazon gebeligin 11., 12., 13. ve 14. gunlerinde hamile farelere verildi. Hayvanlar 12., 13., 14. ve 15. gunlerde kesildi ve fibronektin, pan-kaderin ve beta-katenin poliklonal antikorlari ile boyandi. Bulgularimiz, distal epitelyum, iletici hava kanallari, bazal membranlar, kanallarin lumene bakan yuzeyleri ve mezensim hucrelerinde fibronektin, pan-kaderin ve beta-katenin reaktivitelerinin benzer oldugunu gosterdi. Bu sonuclar, 24 saatlik dogum oncesi deksametazon uygulamalarinin incelenen devrelerde gelisen akciger dokularinda fibronektin, kaderin ve beta-katenin icerikleri uzerine tam bir etkisinin olmadigini dusundurmustur. THE EFFECTS OF GLUCOCORTICOIDS ON THE DISTRIBUTION OF FIBRONECTIN, CADHERIN AND BETA-CATENIN MOLECULES DURING MOUSE LUNG DEVELOPMENT Mammalian lung development involves complex relationships between cells and extracellular matrices. This process mediated by a series of matrix components and adhesion molecules such as fibronectin, laminin, cadherin, catenin and collagen. Glucocorticoids play a central role in fetal lung development. For example it is known that, glucocorticoid administration during pregnancy alters the maturation of the lung. In the present study, we used an immunohistochemical method to evaluate the possible effect of exogenous glucocorticoids to developmental profile of fibronectin, cadherin and betacatenin molecules. Dexamethasone was administrated to pregnant mice on days 11, 12, 13 and 14 of gestation. Animals were sacrificed on days 12, 13, 14 and 15, and stained with polyclonal antibodies to fibronectin, pan-cadherin and beta-catenin. Our results indicated that staining reactivities of fibronectin, pancadherin and beta-catenin were similar in distal epithelia, conductive airways, basal membranes, luminal surfaces of airways and mesenchymal cells. Therefore, we suggest that, 24 hourly antenatal dexamethasone treatments couldn’t completely affect fibronectin, cadherin and beta-catenin contents in evaluated periods of developing lung.