Efficacy of alginate: Nanoliposome encapsulated pediocin against L. innocua in milk system

The present study was carried out to test the efficacy of liposome-alginate-guar gum encapsulated pediocin, a class-II bacteriocin against L. innocua in milk system. Initially, Ultra-turrax homogenizer was used to prepare a coarse emulsion of phospholipid in pediocin solution. Nanoliposomes prepared by high pressure homogenization showed a decrease in liposome size with increase in homogenization pressure and decrease in particle size distribution with increase in number of cycles. The hybrid capsules were compared for its efficacy to deliver pediocin in milk system. However, the encapsulation in alginate as well as in liposomes failed to enhance pediocin activity and inhibit the growth of L. innocua in milk system at both the tested temperatures. Hence, the efficacy of the developed microcapsules can change in different food matrices, which necessitates the microcapsules to be extensively studied for its release mechanics in the respective system before testing against target organisms.