Abstract 12875: Multi-Omics Analysis Strategy is a Promising Approach for Global Identification of New Biomarkers Evaluating Disease Severity of Patients With Dilated Cardiomyopathy

Introduction: Dilated cardiomyopathy (DCM) is an intractable disease and no radical treatment other than cardiac transplantation has been available until today. Because the number of donor heart is limited in Japan, implantation of left ventricular assist device (LVAD) is increasing. Patients can be weaned from LVAD, but no explicit criterion for weaning has been established. Aim: Discovery of novel biomarkers for evaluating severity/recovery of the DCM and biochemical status of the cardiomyocytes. Methods: Multi-omics analyses were performed with left ventricular tissues from heart transplant recipients or autopsy cases with idiopathic DCM and dilated phase of HCM (n=19), and compared with non-heart disease controls (n=8). Proteomic analysis was carried out with a Triple TOF5600 mass spectrometer and quantitatively compared using a 2DICAL software. Transcriptomic and epigenomic (DNA methylation) analysis were performed with Agilent DNA Microarray 44K and Infinium Methylation450 BeadChip kits. Results: 16...