Optimization of Extraction Technique of Astragalus polysaccharide via Response Surface Methodology

The effects of different temperature,time and the ratio of solid to solution on the extraction yield of Astragalus polysaccharide were investigated based on composite design of quadratic regression.The extraction technique parameters were optimized with Response Surface Methodology.Experimental data were analyzed by solving the regression equation with Design MATLAB 7.0software.It was indicated that the optimum extraction parameters were temperature 84℃,time 142minutes and the water volume 276ml.Under those conditions,the predicted value of polysaccharide extraction yield from Radix Astragali was8.97878 μg.mL-1,which was in consistent with the measured value8.945μg.mL-1.It had maximal extraction yield of Astragalus polysaccharide with optimized technique parameters.