Toxicity and anti-inflammatory study of Parmotrema austrosinense extract against oxozalone induced intestinal inflammation in zebrafish (Danio rerio) model

Abstract Lichens are symbiotic organisms producing unique secondary compounds having rich pharmacological value. The aim of the present study is to evaluate the anti-inflammatory potential of lichen Parmotrema austrosinense. Acetone extract of P.austrosinense was evaluated for its anti-inflammatory potential in zebrafish model. In vitro study evidenced the anti-inflammatory activity with inhibitory concentration of 67.43 and 64.68 μg/ml via protease inhibition assay and protein denaturation assay respectively. Toxicity study served as a supporting evidence which revealed that acetone extract of P. austrosinense has no toxicity against tested zebra fishes up to 600 μg/ml and considerable toxicity was observed at 800 μg/ml and 1000 μg/ml. Hence, 200, 400 and 600 μg/ml of acetone extract were used for the treatment of inflammation in fishes. In vivo study evidenced that 70% of the inflammation was cured by 600 μg/ml of acetone extract which in turn confirmed by histopathological evaluation of the intestinal tissues.