Cytotoxic Cytochalasins from Marine-Derived Fungus Arthrinium arundinis

Four new cytochalasins, arthriniumnins A–D ( 1 – 4 ), a new natural product, ketocytochalasin ( 5 ), as well as five known cytochalasin analogues ( 6 – 10 ) were isolated and identified from the fungus Arthrinium arundinis ZSDS1-F3 from the sponge Phakellia fusca . Their structures were elucidated by NMR spectroscopic and mass spectrometric analyses, as well as single crystal X-ray diffraction. Compounds 6 and 9 showed cytotoxicity against K562, A549, Huh-7, H1975, MCF-7, U937, BGC823, HL60, Hela, and MOLT-4 cell lines, with IC 50 values ranging from 1.13 to 47.4 µM.