461 MICRORNA-29 FAMILY AS TUMOR SUPPRESSIVE MICRORNAS IN RENAL CELL CARCINOMA: MICRORNA-29A INHIBITS CELL MIGRATION AND INVASION TARGETING FOCAL ADHESION AND ECM PATHWAYS

limited data exists characterizing whether these changes do exist in ccRCC tissues. METHODS: We therefore undertook a dual analysis of both the metabolome (i.e. small molecules) and the transcriptome in order to comprehensively characterize the metabolism of ccRCC which represents the first such analysis for any tumor type. Small molecule analysis was performed via Gas-Chromatography/ Mass Spectrometry (GC-MS) as well as Liquid-Chromatography/ Mass Spectrometry (LCMS). Transcriptional analysis was performed via mRNA microarray analysis and confirmatory real time RT-PCR. RESULTS: First, our data are the first to validate metabolomics as applied to renal cancer. Second, our data demonstrate significant changes in the transcriptional profile of ccRCC at the levels of glycolysis, gluconeogenesis, and oxidative phosphorylation consistent with a glygolytic phenotype. Third, these changes were consistent with our small molecule analysis and therefore demonstrate a correlation between transcription and metabolic pathway utilization. CONCLUSIONS: Collectively, the data support a unifying model of ccRCC metabolism which further reinforces the association between altered metabolism and malignancy.