Simple and Rapid Isolation of a Novel Antibiotic from Bacillus subtilis Mz‐7

Abstract The difficulties surrounding comparative methods in natural byproduct screening such as the novelty of product, the availability of standards, and the number of trials that can be performed for identification present real challenges to all investigators. A novel antibiotic was isolated and purified from the supernatant of a wild type of Bacillus subtilis Mz‐7 by chloroform precipitation only. The product was checked by TLC and HPLC and proved to be of high purity. Techniques applied in this paper are conveniently reproducible and successfully simplified to isolate and purify an antibiotic, which has the potential to counteract the rise in microbial resistance.