Electrochemical study of a new methylene blue/silicon oxide nanocomposition mediator and its application for stable biosensor of hydrogen peroxide

Abstract A novel organic–inorganic nanocomposite of methylene blue (MB) and silicon oxide was synthesized and characterized by TEM, FTIR, and UV–vis. The as-prepared material was able to transfer the electron of the MB to electrode and was different from other SiO 2 spheres structurally. It can be used as mediator to construct a biosensor with horseradish peroxidase (HRP) coimmobilized in the gelatine matrix and cross-linked with formaldehyde. The resulting biosensor exhibited fast amperometric response and good stability to hydrogen peroxide (H 2 O 2 ). The linear range for H 2 O 2 determination was from 1 × 10 −5 to 1.2 × 10 −3  M, with a detection limit of 4 × 10 −6  M based on S/N = 3. Moreover, the lifetime is more than 3 months under dry conditions at 4 °C.