[Orphanin FQ combined with adriamycin reverses multi-drug resistance of K562/ADM and its molecular mechanism].

Our study have confirmed that orphanin FQ(OFQ) alone can reverse the multi-drug resistance of K562/ADM at the cellular level.Thus,this study was purposed to investigate the molecular mechanism of OFQ combined with ADM that reverses multi-drug resistance of K562/ADM,as well as its correlation with the expression of MDR1 mRNA and P-glycoprotein(P-gp).MTT method was used to detect the proliferation ability of K562/ADM treated with OFQ and ADM alone and their combination;flow cytometry was performed to measure the cell apoptosis rate;real time-PCR was applied to detect the MDR1 mRAN expression;Western blot was used to determine the P-gp expression.The results showed that OFQ(0.1 μmol/L) combined with ADM(15 mg/L) significantly inhibited the cell proliferation of K562/ADM,compared with ADM group;the date gained at 48 h was statistically significant(P0.05),and cell apoptosis rate was significantly raised(P0.01);MDR1 mRNA and P-gp expression levels of OFR combined with ADM were significantly lower than that of ADM alone,and were time-dependent within 48 h.It is concluded that OFQ combined with ADM can reverse the multi-drug resistance of K562/ADM in time-dependent manner,and the 48 h after treatment with these 2 drugs is the best reverse time,which may be related with down regulating the expression of MDR1 mRNA and P-gp.