Effect of lead-excretion glycan on removing lead and essential metal elements in mice

Objective To study the effect of lead-excretion glycan on lead-poisoned mice.Methods 50 healthy mice of Kunming strain(25 males and 25 females) were randomly divided into 5 groups according to their body weights: the negative control group,lead acetate,the lead acetate,model group,the high-dose group(1 600 mg/kg),the medium-dose group(800 mg/kg),and the low-dose group(400 mg/kg).Pb(Ac)2 solutions were given to the groups except the negative control group to form lead-poisoned models in mice,then the low-dose group,the medium-dose group and the high-dose group were given various dose of lead-excretion glycan.The negative control group and the model group were given the normal saline.The mice were sacrificed by decapitation after the last administration.The contents of lead essential elements in blood,liver,kidney,bone and brain were determined by graphite furnace atomic absorption spectrophotometer.Results The contents of lead in blood,liver,kidney,bone and brain in the treated groups were all lower than that in the model group(P0.05).Magnesiumcontents of the high-dose group in blood and bone were lower than that of the negative control group(P0.05).Iron contents of the medium and high dose groups in liver was higher than that of model group(P0.05).There was no statistically significant difference between the negative group and various dose groups in the content of calcium,copper zinc and manganese in liver,kidney,bone and brain(P0.05).Conclusion Lead-excretion glycan can effectively eliminate lead from lead-poisoned mice,but there is no significant dose-effect relationship.Lead-excretion glycan has no effect on other essential metal elements except that the high dose glycan can eliminate magnesium from blood and bone.The medium and high dose glycan can protect iron in the liver.