Heavy chain variable and constant region alloytpes in single rabbit plasma cells

Abstract Spleen cells from rabbits heterozygous for both a variable region (group a ) allotype and an allotype characteristic of the constant region of IgG (group d and group e ) or the constant region of IgM ( Ms 4 or Ms 6) were examined by double staining with fluroescent antiallotype sera. The results indicate that one chromosome may a variable regio gene while its homologous chromosome may contribute a constant region gene to the synthesis of a single H chain. Such an interaction appears to be responsible for the synthesis of about per cent of the γ and μ chains.