Study on Transdermal Absorption of Praziquantel Solution at High Concentration and Its Worm-Reduction Effect on Schistosomiasis Japonicum in Mice

OBJECTIVE To prepare praziquantel solution at high concentration and to investigate its transdermal delivery and worm-reduction effect on Schistosomiasis japoricum in mice.METHODS The stability of the solution was evaluatad by experiments.The solubility of praziquantel solution at different temperature was measured by UV spectrophotometer.The praziquantel concentrations in rabbit plasma were determined by HPLC.The praziquantel solution of 70,150,200 g·L2-1,were applied to the experimental therapy of Schistosomiasis japonicum in mice.RESULTS The praziquantel solution at high concentration showed good stability.From 35 to 45 ℃, there was a great change in the solubility of praziquantel in the solvent(284 g·L2-1→456 g·L2-1).The absorption rate of transdermal delivery was faster than that of oral administration.The AUC and ρ2max of the former was as 2.1 times and 4.0 times as oral administration.The praziquantel solution reached prefect therapy efficacy with the worm-reduction of 100%. CONCLUSION The solution containing high praziquantel concentration may be a promising vehicle for the transdermal delivery of praziquantel.