hGH ASSAY IN URINE: PRELIMINARY RESULTS OF A FRENCH MULTI-CENTRIC EVALUATION
1993
Urines hGH was determined using E.I.A. commercial kit (NORDITEST) from NOVO NORDISK A/S. Inter-laboratory quality controls were performed in urines samples and standard calibrators: C.V. were between 18.5-29% at low concentrations (about 5 ng/l) and 10-15% for higher concentrations (about 20 ng/l). First morning urine samples were collected for 3 nights in 227 children (122 boys and 105 girls) mean age 9.0 (range 4 to 15 years) using plastic containers after addition of BSA solution (30% w/v). The following groups were studied: normal subjects (A), short stature with GH deficiency after pharmacological tests (B) and without GHD (C), nephrotic syndrome (D), diabetes mellitus (E), obesity (F), growth advance untreated (G) and somatostation treated (H). The results expressed in u-hGH ng/mmol of creatinine, were:
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